neuronal p2x7 receptor induced reactive oxygen species production contributes to nociceptive behavior in mice

Intrathecal administration of BzATP resulted in ROS production in the spinal cord and oxidative DNA damage in dorsal horn neurons. To confirm CellROX intensity increased due to ROS production, cells were pre-treated with the ROS scavenger N-tert-butyl-α-phenylnitrone (PBN, 30 µM) for 30 min. ATP induces ROS increase in spinal cord dorsal horn (SCDH) neurons. About...